FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8898
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC260329.253764890986737No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC235826.500337154416727No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC94610.631602607327489No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG6347.1251966734097545No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC4985.596763317599461No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1181.326140705776579No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA530.5956394695437177No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.5169701056417173No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA430.48325466396943134No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG360.4045853000674309No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.26972353337828725No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.24724657226342997No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.23600809170600134No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.23600809170600134No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.23600809170600134No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.22476961114857275No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.22476961114857275No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.21353113059114406No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.20229265003371544No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA180.20229265003371544No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1910541694762868No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1910541694762868No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1910541694762868No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1910541694762868No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1910541694762868No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.16857720836142953No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1573387278040009No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.14610024724657228No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14610024724657228No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.14610024724657228No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.13486176668914363No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.12362328613171499No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.12362328613171499No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC100.11238480557428637No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT100.11238480557428637No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.10114632501685772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)