FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6614
Total Bases958.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC198429.996976111279107No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC135820.532204414877533No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG87713.259752041124887No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC76711.596613244632598No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2353.5530692470517087No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG741.1188388267311764No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA550.8315693982461446No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.4535833081342607No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT290.4384638645297853No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.2872694284850318No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.2721499848805564No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC180.2721499848805564No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT160.24191109767160568No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.24191109767160568No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.22679165406713034No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.21167221046265497No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.21167221046265497No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.21167221046265497No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.19655276685817963No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.19655276685817963No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.18143332325370426No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.18143332325370426No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.1663138796492289No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA110.1663138796492289No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC110.1663138796492289No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.15119443604475355No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC100.15119443604475355No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.1360749924402782No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTCGCAAATTGCTGCTGGAACC90.1360749924402782No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.1360749924402782No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.1360749924402782No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC80.12095554883580284No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT80.12095554883580284No Hit
AAATACCGTTCAGAGCTGCCCACAGAAACACACACACAACCGAATGTAGA80.12095554883580284No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.12095554883580284No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.12095554883580284No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.10583610523132748No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.10583610523132748No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.10583610523132748No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.10583610523132748No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT70.10583610523132748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)