FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10096
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG473346.87995245641838No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC170616.897781299524564No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT110510.944928684627575No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC8628.538034865293186No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC1891.8720285261489698No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA690.6834389857369255No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC570.5645800316957211No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG530.5249603803486529No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT430.4259112519809826No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC400.39619651347068147No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC290.28724247226624405No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG260.25752773375594296No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG240.2377179080824089No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT230.22781299524564183No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCGT170.16838351822503964No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC170.16838351822503964No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC170.16838351822503964No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG150.14857369255150554No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.1386687797147385No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT130.12876386687797148No Hit
TGTGTGTGTGTTTCTGTGGGCTTCTTTAAGGTTTGGACAGAAGGGTAAAG130.12876386687797148No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.11885895404120445No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT120.11885895404120445No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.11885895404120445No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG120.11885895404120445No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTGTGTGAAGACCAAGTCTCT120.11885895404120445No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC110.1089540412044374No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT110.1089540412044374No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC110.1089540412044374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)