FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10096
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC408040.41204437400951No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC160015.847860538827257No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC103810.281299524564185No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG8488.399366085578446No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC3343.3082408874801903No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1861.8423137876386688No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA710.7032488114104596No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA680.6735340729001585No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.4358161648177496No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.4259112519809826No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG390.38629160063391443No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.33676703645007927No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA320.31695721077654515No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.31695721077654515No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.28724247226624405No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT280.277337559429477No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.26743264659271No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.25752773375594296No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2377179080824089No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA230.22781299524564183No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT230.22781299524564183No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.2179080824088748No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1881933438985737No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC160.15847860538827258No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.14857369255150554No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.1386687797147385No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.1386687797147385No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC140.1386687797147385No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT120.11885895404120445No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT120.11885895404120445No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA110.1089540412044374No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC110.1089540412044374No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC110.1089540412044374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)