FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19894
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC499725.118126068161256No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC480224.137931034482758No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC336616.919674273650347No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG214910.80225193525686No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG5572.7998391474816526No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA1330.6685432793807178No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1100.5529305318186388No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA820.4121845782648035No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT820.4121845782648035No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGTTGCTGGAACC520.26138534231426563No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.24630541871921183No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT460.23122549512415805No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT450.22619885392580677No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT440.22117221272745552No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.20609228913240174No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.1809590831406454No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA340.1709058007439429No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.1709058007439429No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.16587915954559163No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.16085251834724038No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.16085251834724038No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT310.1558258771488891No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.1558258771488891No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.1457725947521866No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA290.1457725947521866No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT290.1457725947521866No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.14074595355383532No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.14074595355383532No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.14074595355383532No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.13571931235548407No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.13069267115713282No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.12566602995878154No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT250.12566602995878154No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC250.12566602995878154No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT230.11561274756207902No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT230.11561274756207902No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.11058610636372776No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA210.1055594651653765No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.1055594651653765No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT200.10053282396702524No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT200.10053282396702524No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT200.10053282396702524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)