FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13936
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC274319.682835820895523No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC240317.243111366245696No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTTTCAATTAAATACAA190413.662456946039036No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC180512.952066590126291No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG148010.619977037887486No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG3762.69804822043628No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2041.4638346727898968No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1320.947187141216992No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1010.7247416762342135No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC890.638633754305396No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA690.49512055109070036No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.30855338691159584No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA430.30855338691159584No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT420.30137772675086105No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT350.2511481056257176No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTTTCAATTAAATACAAT330.236796785304248No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT320.2296211251435132No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.20809414466130882No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTTTCAATTAAATACAAT270.19374282433983928No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTTTCAATTAAATACAAT260.18656716417910446No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.1793915040183697No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT250.1793915040183697No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC230.16504018369690013No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA210.15068886337543053No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT200.14351320321469577No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT200.14351320321469577No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.13633754305396095No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1291618828932262No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.12198622273249138No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.12198622273249138No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.12198622273249138No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT160.1148105625717566No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTTTCAATTAAATACAAT160.1148105625717566No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.1076349024110218No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA150.1076349024110218No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT150.1076349024110218No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTTTCAATTAAATACAAT150.1076349024110218No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1076349024110218No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1076349024110218No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.10045924225028703No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTTTCAATTAAATACAAT140.10045924225028703No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.10045924225028703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)