FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17557
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC582533.17764994019479No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC332418.932619468018455No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG195211.118072563649827No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC13117.467107136754571No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC5653.2180896508515118No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG4662.654211995215584No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1740.9910576977843595No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA1270.7233582047046762No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1220.6948795352281142No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC1190.6777923335421769No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1080.6151392606937405No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT810.4613544455203053No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA660.3759184370906191No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA630.35883123540468187No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT510.29048242866093293No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.2676994930796833No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA450.2563080252890585No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA450.2563080252890585No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA420.23922082360312127No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT400.22782935581249644No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT380.21643788802187164No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.2050464202312468No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.1993506863359344No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.1993506863359344No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.17656775075468475No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT300.17087201685937234No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA290.16517628296405992No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.13669761348749787No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA220.12530614569687304No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.12530614569687304No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.12530614569687304No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.11961041180156064No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC190.10821894401093582No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC180.1025232101156234No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC180.1025232101156234No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.1025232101156234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)