FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576
Total Bases518.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC74220.74944071588367No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC65718.37248322147651No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG46513.003355704697986No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC40711.381431767337807No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC3469.675615212527964No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1273.551454138702461No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG471.3143176733780761No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT300.8389261744966443No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.47539149888143173No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA160.44742729306487694No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.3635346756152125No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.33557046979865773No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT110.3076062639821029No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2796420581655481No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2796420581655481No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.25167785234899326No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.25167785234899326No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.25167785234899326No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.22371364653243847No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.22371364653243847No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.22371364653243847No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.22371364653243847No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.19574944071588368No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT70.19574944071588368No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.13982102908277405No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.13982102908277405No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.13982102908277405No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.13982102908277405No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.13982102908277405No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.13982102908277405No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC50.13982102908277405No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.13982102908277405No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCACATTATTTCAATTAAATAC40.11185682326621924No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.11185682326621924No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.11185682326621924No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGGACC40.11185682326621924No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.11185682326621924No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.11185682326621924No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA40.11185682326621924No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.11185682326621924No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT40.11185682326621924No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT40.11185682326621924No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC40.11185682326621924No Hit
GGCACTAGAATGGACCTCTTCCTCATCAATACATTATTTCAATTAAATAC40.11185682326621924No Hit
GGCACTAGAATGGACCTCTCCTCATCACTACATTATTTCAATTAAATACA40.11185682326621924No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.11185682326621924No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC40.11185682326621924No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTACAAATTGCTGCTGGAACC40.11185682326621924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)