FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5292
Total Bases767.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT193936.64021164021164No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG160630.347694633408917No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC61411.60241874527589No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC1572.9667422524565383No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG601.1337868480725624No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC591.1148904006046865No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC500.944822373393802No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG440.8314436885865457No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA320.6046863189720333No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC250.472411186696901No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC230.4346182917611489No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG210.3968253968253968No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCGT200.3779289493575208No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.22675736961451248No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.22675736961451248No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT100.1889644746787604No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT90.17006802721088435No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT90.17006802721088435No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTGTGTGAAGACCAAGTCTCT80.15117157974300832No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.15117157974300832No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTGTGTGAAGACCAAGTCTCT80.15117157974300832No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT70.13227513227513227No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTGTGTGAAGACCAAGTCTCT70.13227513227513227No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.13227513227513227No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC70.13227513227513227No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT60.11337868480725624No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.11337868480725624No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG60.11337868480725624No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT60.11337868480725624No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG60.11337868480725624No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.11337868480725624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)