FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301
Total Bases478.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC103831.445016661617693No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC64719.600121175401394No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG43013.02635564980309No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2868.664041199636474No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1013.0596788851863073No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG932.817328082399273No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG320.9694032111481369No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT190.5755831566192063No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.45440775522568916No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA150.45440775522568916No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.3938200545289306No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.3029385034837928No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.3029385034837928No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC100.3029385034837928No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT90.27264465313541353No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.24235080278703422No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT80.24235080278703422No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT70.21205695243865494No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA70.21205695243865494No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.21205695243865494No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.21205695243865494No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC60.18176310209027569No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.18176310209027569No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT60.18176310209027569No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.1514692517418964No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT40.12117540139351711No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.12117540139351711No Hit
AAATACCGTTCAGAGCTGCCACCAAGGGCTTGCAAATTGCTGCTGGAACC40.12117540139351711No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTAGCAAATTGCTGCTGGAACC40.12117540139351711No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC40.12117540139351711No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.12117540139351711No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.12117540139351711No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC40.12117540139351711No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)