FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10938
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC307428.10385810934357No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC143113.08283049917718No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC138512.662278295849333No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG124911.418906564271348No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC116910.687511428049005No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG3052.788443956847687No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1070.9782409946973852No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG670.6125434265862132No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA550.5028341561528615No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA500.45712196013896506No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT440.4022673249222892No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2925580544889376No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT310.2834156152861584No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT290.26513073688059974No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.25598829767782044No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.23770341927226182No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.23770341927226182No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.22856098006948253No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.21941854086670326No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC230.21027610166392394No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT230.21027610166392394No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT220.2011336624611446No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.19199122325836532No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.19199122325836532No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.19199122325836532No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.17370634485280673No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.16456390565002743No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA170.15542146644724814No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT160.1462790272444688No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.13713658804168952No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.12799414883891022No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.12799414883891022No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.11885170963613091No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.11885170963613091No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.11885170963613091No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10970927043335163No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.10970927043335163No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1005668312305723No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1005668312305723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)