FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015
Total Bases582 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC115228.692403486924036No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC91522.789539227895393No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC44911.183063511830635No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG42810.660024906600249No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1172.9140722291407224No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC441.095890410958904No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG411.021170610211706No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA220.547945205479452No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC210.523038605230386No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.34869240348692404No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.34869240348692404No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.32378580323785805No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT130.32378580323785805No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT110.273972602739726No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.24906600249066002No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA100.24906600249066002No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.24906600249066002No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.199252801992528No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.199252801992528No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.199252801992528No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT80.199252801992528No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.17434620174346202No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.17434620174346202No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA70.17434620174346202No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.17434620174346202No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.17434620174346202No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.17434620174346202No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT70.17434620174346202No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC60.14943960149439603No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.14943960149439603No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.14943960149439603No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.14943960149439603No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.14943960149439603No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.12453300124533001No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.12453300124533001No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC50.12453300124533001No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.12453300124533001No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT50.12453300124533001No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC50.12453300124533001No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGACCCATG50.12453300124533001No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.12453300124533001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)