FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12284
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC392931.984695538912405No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC197616.085965483555846No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC196215.971996092478019No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG142511.600455877564311No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG4063.3051123412569194No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG810.6593943340931292No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT780.6349723217193096No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA730.5942689677629437No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA610.4965809182676652No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.35818951481602085No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT420.34190817323347444No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.284923477694562No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.26864213611201565No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2605014653207424No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2605014653207424No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.2523607945294692No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT290.23607945294692284No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT280.22793878215564964No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT240.19537609899055683No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA230.1872354281992836No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.17909475740801042No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA220.17909475740801042No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC220.17909475740801042No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.17095408661673722No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT200.16281341582546402No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.14653207424291761No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1383914034516444No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA170.1383914034516444No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.1302507326603712No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.11396939107782482No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC140.11396939107782482No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC140.11396939107782482No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.11396939107782482No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT140.11396939107782482No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.1058287202865516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)