FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4194
Total Bases607.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC140333.45255126371006No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC113026.94325226514068No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG58213.876967095851217No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1794.2680019074868865No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG300.7153075822603719No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.5722460658082975No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.5007153075822603No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.5007153075822603No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA190.45302813543156895No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA180.4291845493562232No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.35765379113018597No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.35765379113018597No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.33381020505484027No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT130.3099666189794945No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.3099666189794945No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.28612303290414876No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.28612303290414876No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.26227944682880305No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.26227944682880305No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.26227944682880305No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.2384358607534573No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2384358607534573No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC90.2145922746781116No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT90.2145922746781116No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT80.19074868860276584No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.19074868860276584No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA70.16690510252742013No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.16690510252742013No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.14306151645207438No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC60.14306151645207438No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA60.14306151645207438No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTACTGGAACC60.14306151645207438No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCCGGAACC60.14306151645207438No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.11921793037672865No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC50.11921793037672865No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATCGCTGCTGGAACC50.11921793037672865No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC50.11921793037672865No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)