FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463
Total Bases212 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC32222.00956937799043No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC23816.267942583732058No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC22115.105946684894054No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG20814.21736158578264No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC875.946684894053315No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG573.896103896103896No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG241.6404647983595353No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT151.0252904989747096No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA80.5468215994531784No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.4101161995898838No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT60.4101161995898838No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT60.4101161995898838No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.3417634996582365No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.2734107997265892No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.2050580997949419No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.2050580997949419No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.2050580997949419No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCCTGCAAATTGCTGCTGGAACC30.2050580997949419No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.2050580997949419No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.2050580997949419No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.2050580997949419No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTACCGAATGTAGAACCATGT30.2050580997949419No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.2050580997949419No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC30.2050580997949419No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.1367053998632946No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC20.1367053998632946No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1367053998632946No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1367053998632946No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.1367053998632946No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.1367053998632946No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGATTGCAAATTGCTGCTGGAACC20.1367053998632946No Hit
AAATACCGTTCAGAGCTGCCACCAAGCGCTTGCAAATTGCTGCTGGAACC20.1367053998632946No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1367053998632946No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC20.1367053998632946No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT20.1367053998632946No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.1367053998632946No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC20.1367053998632946No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACAC20.1367053998632946No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC20.1367053998632946No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGAAAATTGCTGCTGGAACC20.1367053998632946No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1367053998632946No Hit
GGCACTAGAATGGACCTCTTCCCACCCAAACCCACAGAAACACACACACA20.1367053998632946No Hit
GGCACTAGAATGGACCTTTCCTCATCACTACATTATTTCAATTAAATACA20.1367053998632946No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC20.1367053998632946No Hit
GGCACTAGAATGGACCTTCCTCATCACTACATTATTTCAATTAAATACAA20.1367053998632946No Hit
AAATACTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGTCG20.1367053998632946No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTCCAATTAAATAC20.1367053998632946No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC20.1367053998632946No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.1367053998632946No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT20.1367053998632946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)