FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306
Total Bases479.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT170051.421657592256494No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG50715.335753176043557No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC45113.641863278886873No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC812.4500907441016335No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG411.2401693889897156No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC330.9981851179673321No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC280.8469449485783425No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG190.5747126436781609No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA190.5747126436781609No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.3629764065335753No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC110.33272837265577737No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT60.18148820326678766No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT60.18148820326678766No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTACCT60.18148820326678766No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.18148820326678766No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.18148820326678766No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.1512401693889897No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTGTGTGAAGACCAAGTCTCT50.1512401693889897No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.1512401693889897No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT50.1512401693889897No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.1512401693889897No Hit
ACTTAAAAACAAGGCAGTGCTAACGCCTAATGGTGCTACAGTTTCTGCCT50.1512401693889897No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT50.1512401693889897No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT50.1512401693889897No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT40.12099213551119178No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTGTGTGAAGACCAAGTCTCT40.12099213551119178No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG40.12099213551119178No Hit
ACTTAAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCC40.12099213551119178No Hit
ACTTAAAAACAAGGCAGTGCCAATGCCTAATGGTGCTACAGTTTCTGCCT40.12099213551119178No Hit
TGTGTGTGTGTTTCTGTGGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAA40.12099213551119178No Hit
ACTTAAAAACAAGGCAGGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.12099213551119178No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC40.12099213551119178No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAACGGTGCTACAGTTTCTGCCT40.12099213551119178No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)