FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141
Total Bases455.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC103632.98312639286851No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC78424.960203756765363No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG51116.268704234320282No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1123.565743393823623No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG290.9232728430436167No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA250.795924864692773No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.7322508755173511No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.5730659025787965No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.5093919134033748No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.44571792422795287No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.38204393505253104No Hit
AAATACCGTTCAGAGCTGCCACTAGGAGCTTGCAAATTGCTGCTGGAACC120.38204393505253104No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC120.38204393505253104No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.3183699458771092No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT100.3183699458771092No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.28653295128939826No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.28653295128939826No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT90.28653295128939826No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA80.2546959567016874No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.2546959567016874No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.2546959567016874No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA70.22285896211397643No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.22285896211397643No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.22285896211397643No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT60.19102196752626552No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTACAAATTGCTGCTGGAACC60.19102196752626552No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC60.19102196752626552No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT60.19102196752626552No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.1591849729385546No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.1591849729385546No Hit
GGCACTAGAATGGACCTCTTCCTCATCACAACATTATTTCAATTAAATAC50.1591849729385546No Hit
AAATACCGTTCAGGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.1591849729385546No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC40.1273479783508437No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.1273479783508437No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC40.1273479783508437No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGACCGAATGTAGAACCATGT40.1273479783508437No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT40.1273479783508437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)