FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233
Total Bases178.7 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC35829.034874290348746No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC26821.73560421735604No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1169.407948094079481No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC1098.840227088402271No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC977.86699107866991No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG252.02757502027575No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT181.4598540145985401No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.5677210056772101No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.48661800486618007No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG60.48661800486618007No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.40551500405515No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.32441200324412006No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTACAAATTGCTGCTGGAACC40.32441200324412006No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.32441200324412006No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.32441200324412006No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT40.32441200324412006No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT40.32441200324412006No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.24330900243309003No Hit
AAAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC30.24330900243309003No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.24330900243309003No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT30.24330900243309003No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA30.24330900243309003No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA30.24330900243309003No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.24330900243309003No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC30.24330900243309003No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.24330900243309003No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTACAAATTGCTGCTGGAACCT20.16220600162206003No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.16220600162206003No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.16220600162206003No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.16220600162206003No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.16220600162206003No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTACTTCAATTAAATAC20.16220600162206003No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.16220600162206003No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC20.16220600162206003No Hit
AAATACCGTTCAGAGCTGCCACCAAGAACTTGCAAATTGCTGCTGGAACC20.16220600162206003No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC20.16220600162206003No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC20.16220600162206003No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.16220600162206003No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC20.16220600162206003No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC20.16220600162206003No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC20.16220600162206003No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT20.16220600162206003No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.16220600162206003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)