FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2920
Total Bases423.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC80827.671232876712327No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG60420.684931506849317No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC44015.068493150684931No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC2117.226027397260274No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1655.650684931506849No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG220.7534246575342466No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA200.684931506849315No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.4452054794520548No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT130.4452054794520548No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.3082191780821918No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT90.3082191780821918No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT80.273972602739726No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.273972602739726No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.273972602739726No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.2397260273972603No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.2397260273972603No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.2397260273972603No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.2397260273972603No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.2397260273972603No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.2397260273972603No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.2397260273972603No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT70.2397260273972603No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT60.2054794520547945No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.2054794520547945No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.2054794520547945No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT60.2054794520547945No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.2054794520547945No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT60.2054794520547945No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.17123287671232876No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.17123287671232876No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.17123287671232876No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.17123287671232876No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.17123287671232876No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTACCGAATGTAGAACCATGT50.17123287671232876No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.17123287671232876No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT50.17123287671232876No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAGCC40.136986301369863No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGCACC40.136986301369863No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.136986301369863No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT40.136986301369863No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.136986301369863No Hit
GGCACTAGAATGGACCTCTTCAGAGCACTGCCTTGTTTTTAAGTACCGAA40.136986301369863No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.136986301369863No Hit
GGCACTAGAATGGACCTCTTCTTCATCACTACATTATTTCAATTAAATAC40.136986301369863No Hit
AAATACCGTTCAGAGCTGCCCACAGAAACACACACACAACCGAATGTAGA40.136986301369863No Hit
GGCACTAGAATGGATCTCTTCCTCATCACTAAATTATTTCAATTAAATAC40.136986301369863No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC40.136986301369863No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC30.10273972602739725No Hit
GGCACTAGAATGGACCTCTTCAGAGCAACTCTGCGTTGATACCACATGAT30.10273972602739725No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACA30.10273972602739725No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAGATTGCTGCTGGAACC30.10273972602739725No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.10273972602739725No Hit
AAATACCGTTCAGAGCTGCCACCGGGAGCTTGCAAATTGCTGCTGGAACC30.10273972602739725No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTACTGGAACC30.10273972602739725No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC30.10273972602739725No Hit
GGCACTAGAATGGACCTCTTCAGAGCAACTCTGCGTTGATAGCACTGCCT30.10273972602739725Clontech Universal Primer Mix Long (95% over 21bp)
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC30.10273972602739725No Hit
AAATACCGTTCAGGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT30.10273972602739725No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10273972602739725No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTACATTATTTCAATTAAATAC30.10273972602739725No Hit
AAATACCGTTCAGAGCTGCCACCCACAGAAACACACACACAACCGAATGT30.10273972602739725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)