FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439
Total Bases208.6 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC85159.13829047949966No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC23316.191799861014594No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC584.030576789437109No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA271.8763029881862403No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT221.5288394718554552No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT201.389854065323141No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT140.9728978457261988No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCGT130.9034051424600417No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTGTGTGAAGACCAAGTCTCT70.4864489228630994No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTGTGTGAAGACCAAGTCTCT70.4864489228630994No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTGTGTGAAGACCAAGTCTCT60.4169562195969423No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTGTGTGAAGACCAAGTCTCT50.34746351633078526No Hit
ACTTAAAAACAAGGCAGCCTGAACGGTATTTGTGTGAAGACCAAGTCTCT50.34746351633078526No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC40.27797081306462823No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT30.20847810979847115No Hit
ACTTAAAAACAAGGCAGCTTGAACGGTATTTGTGTGAAGACCAAGTCTCT30.20847810979847115No Hit
TGTGTGTGTGTTTCTGTAGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC30.20847810979847115No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTGTGTGAAGACCAAGTCTCT30.20847810979847115No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCGT30.20847810979847115Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAGGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC30.20847810979847115No Hit
ACTTAAAAACAAGGCAGTTCGAACGGTATTTGTGTGAAGACCAAGTCTCT30.20847810979847115No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGATTTGTGTGAAGACCAAGTCTCT30.20847810979847115No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT30.20847810979847115No Hit
ACTTAAAAACAAGGCAGTTCTGACGGTATTTGTGTGAAGACCAAGTCTCT30.20847810979847115No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT30.20847810979847115No Hit
TGTGTGTGTGTTTCTGAACGGTATTTGTGTGAAGACCAAGTCTCTGCTAC20.13898540653231412No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTGTGTGAAGACCAAGTCTCT20.13898540653231412No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG20.13898540653231412No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTGTGTGAAGACCAAGTCTCT20.13898540653231412No Hit
ACTTAAAAACAAGGCGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.13898540653231412No Hit
TGTGTGTGAAGACCAAGTCTCTGCTACCGTATTGCACTCAGATCTCGTAT20.13898540653231412No Hit
ACTTAAAAACAAGGCAGTGCTGAACGATATTTGTGTGAAGACCAAGTCTC20.13898540653231412No Hit
TGTGTGTGTGTTTCTGTGGGTGGCAGCTCTGAACGGTATTTGTGTGAAGA20.13898540653231412No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.13898540653231412No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATATGTGTGAAGACCAAGTCTC20.13898540653231412No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGCCCAAGTCTC20.13898540653231412No Hit
ACTTAAAAAAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.13898540653231412No Hit
ACTTAAAAACAAGGCAGTGCTGAAGGTATTTGTGTGAAGACCAAGTCTCT20.13898540653231412No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTTTTGTGTGAAGACCAAGTCTCT20.13898540653231412No Hit

[FAIL]Adapter Content

Adapter graph