FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286
Total Bases186.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG50439.19129082426128No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC26320.45101088646967No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT17413.530326594090203No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC896.920684292379471No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC211.6329704510108864No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA141.088646967340591No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT100.7776049766718507No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC80.6220839813374806No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.6220839813374806No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTTGCTAA70.5443234836702955Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGTGCTAAGAGGTCCATTCTAGTGCCGTGTGAAGA70.5443234836702955No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.5443234836702955No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTACATGG70.5443234836702955Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.46656298600311047No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTTGCGAA50.38880248833592534Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG40.3110419906687403No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTGTGTGAAGACCAAGTCTCT30.23328149300155523No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG30.23328149300155523No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTTGAAGA30.23328149300155523Clontech Universal Primer Mix Long (96% over 27bp)
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG20.15552099533437014No Hit
TGTGTGTGTGTTTCTGTGGGCTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.15552099533437014No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.15552099533437014No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.15552099533437014No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.15552099533437014No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG20.15552099533437014No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAGTGGTGCTACAGTTTCTGCCTC20.15552099533437014No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.15552099533437014No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.15552099533437014No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.15552099533437014No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT20.15552099533437014No Hit
TGTGTGTGTGTTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.15552099533437014No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG20.15552099533437014No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.15552099533437014No Hit
ACTTAAAAACAAGGCAGTGCGAAGAGGTCCATTCTAGTGCCGTGTGAAGA20.15552099533437014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)