FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819
Total Bases553.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC67017.543859649122805No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC65917.255826132495418No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC57915.161036920659859No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC51613.511390416339356No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG44311.599895260539409No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG761.9900497512437811No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT591.544907043728725No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT350.9164702801780571No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA300.7855459544383346No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG260.6808064938465567No Hit
GGCACTAGAATGGACCTCTTCTGACCCGGCCCAAGCAATCACTCCCTAGT160.41895784236711187No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.3927729772191673No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.3142183817753339No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.2880335166273894No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT100.2618486514794449No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2618486514794449No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.2356637863315004No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.2356637863315004No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.20947892118355593No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.20947892118355593No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA80.20947892118355593No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.20947892118355593No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC80.20947892118355593No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.1832940560356114No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA70.1832940560356114No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.1832940560356114No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA70.1832940560356114No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.15710919088766695No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.13092432573972246No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.13092432573972246No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.13092432573972246No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.13092432573972246No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.13092432573972246No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.13092432573972246No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.13092432573972246No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.13092432573972246No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTTCTGCTGGAACC50.13092432573972246No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.13092432573972246No Hit
AAATCCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.10473946059177797No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT40.10473946059177797No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.10473946059177797No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC40.10473946059177797No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC40.10473946059177797No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.10473946059177797No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.10473946059177797No Hit
AAATACCGTTCAGAGCTGCCCACAGAAACACACACACAACCGAATGTAGA40.10473946059177797No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.10473946059177797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)