FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8512
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC320437.640977443609025No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC187922.074718045112782No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG7879.245770676691729No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC6077.131109022556391No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2412.831296992481203No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA630.7401315789473684No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG530.6226503759398496No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.4464285714285714No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.4346804511278195No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT340.39943609022556387No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA320.37593984962406013No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.30545112781954886No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT250.29370300751879697No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA240.28195488721804507No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.24671052631578946No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.24671052631578946No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC200.2349624060150376No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.2349624060150376No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.2232142857142857No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.21146616541353383No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA170.19971804511278193No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.18796992481203006No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT150.1762218045112782No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC150.1762218045112782No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT140.1644736842105263No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA130.15272556390977443No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC130.15272556390977443No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.14097744360902253No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.12922932330827067No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT110.12922932330827067No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC100.1174812030075188No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.1174812030075188No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.1174812030075188No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.1174812030075188No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT100.1174812030075188No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC90.10573308270676691No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.10573308270676691No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACC90.10573308270676691No Hit
GGCACTAGAATGGACCTTTCCTCATCACTACATTATTTCAATTAAATACA90.10573308270676691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)