FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4023
Total Bases583.1 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC100925.08078548347005No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC89622.271936365896096No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG61115.18767089236888No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC4019.967685806611982No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1393.4551329853343278No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC1333.3059905543127024No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG390.9694258016405668No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.49714143673875216No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA170.4225702212279393No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.4225702212279393No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.3479990057171265No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.3231419338801889No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC120.2982848620432513No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.2734277902063137No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.24857071836937608No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT100.24857071836937608No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA90.22371364653243847No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.19885657469550086No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.19885657469550086No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT80.19885657469550086No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.19885657469550086No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.19885657469550086No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.17399950285856325No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.17399950285856325No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA70.17399950285856325No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT70.17399950285856325No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC60.14914243102162564No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGATTGCAAATTGCTGCTGGAACC60.14914243102162564No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.14914243102162564No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.14914243102162564No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.12428535918468804No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.12428535918468804No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.12428535918468804No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.12428535918468804No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA50.12428535918468804No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.12428535918468804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)