FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5053
Total Bases732.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC127325.192954680387885No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC76315.099940629329112No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG62912.448050662972491No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC61212.111616861270532No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC54110.706510983574114No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1452.869582426281417No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT591.1676231941420938No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT440.8707698396991885No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG300.5937067088858103No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT260.5145458143677024No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA250.4947555907381753No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC180.3562240253314862No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.23748268355432417No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.21769245992479713No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT110.21769245992479713No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.19790223629527012No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.19790223629527012No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT100.19790223629527012No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.19790223629527012No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.1781120126657431No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.1781120126657431No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC80.1583217890362161No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.1385315654066891No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.1385315654066891No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.1385315654066891No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.1385315654066891No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.1385315654066891No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.1385315654066891No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.1385315654066891No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT60.11874134177716208No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC60.11874134177716208No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.11874134177716208No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.11874134177716208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)