FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10779
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC396636.793765655441135No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC196718.24844605250951No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG111010.297801280267187No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC5945.510715279710548No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC4073.7758604694313016No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2512.3286019111234806No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1851.7163002133778644No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1141.057612023378792No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA700.6494108915483812No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA650.6030243992949254No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG590.5473606085907783No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.40820113183041096No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.3989238333797198No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.3803692364783375No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.3339827442248817No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.3339827442248817No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT290.2690416550700436No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.2690416550700436No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.25976435661935243No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.23193246126727898No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.23193246126727898No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC250.23193246126727898No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.18554596901382317No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.18554596901382317No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC200.18554596901382317No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT170.1577140736617497No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.13915947676036738No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA150.13915947676036738No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.12988217830967622No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT140.12988217830967622No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC120.11132758140829391No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC120.11132758140829391No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC110.10205028295760274No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC110.10205028295760274No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10205028295760274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)