FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14702
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC412228.037001768466872No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC377225.65637328254659No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG150610.243504285131275No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC14629.944225275472725No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG3112.1153584546320228No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2701.8364848319956466No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1501.0202693511086927No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1290.8774316419534757No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA1160.7890082981907224No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC800.5441436539246362No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA670.45572031016188275No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA590.4013059447694191No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT590.4013059447694191No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.23126105291797036No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.20405387022173854No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.20405387022173854No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA300.20405387022173854No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.20405387022173854No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT290.1972520745476806No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT270.18364848319956467No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.17684668752550675No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT250.17004489185144878No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC250.17004489185144878No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC230.15644130050333288No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.15644130050333288No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.14963950482927493No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.14963950482927493No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.14963950482927493No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT210.14283770915521696No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.14283770915521696No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA200.13603591348115904No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.12923411780710106No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.12923411780710106No Hit
GGCACTAGAATGGACCTCTTCCCACCCAAACCCACAGAAACACACACACA190.12923411780710106No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA180.12243232213304313No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.11563052645898518No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC160.10882873078492722No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.10202693511086927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)