FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6316
Total Bases915.2 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC441169.83850538315389No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG4497.108929702343255No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1332.1057631412286257No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA941.4882837238758708No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA540.8549715009499683No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.6649778340721976No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC410.64914502849905No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.6016466117796073No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG300.4749841671944269No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.4749841671944269No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.4591513616212793No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.44331855604813175No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.44331855604813175No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.41165294490183657No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA240.37998733375554145No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC240.37998733375554145No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.31665611146295125No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA170.26915769474350854No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.26915769474350854No Hit
GGCACTAGAATGGACCTTCCTCATCACTAAATTATTTCAATTAAATACAA140.22165927802406588No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA140.22165927802406588No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTAAATTATTTCAATTAAATAC120.18999366687777072No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC120.18999366687777072No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC120.18999366687777072No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC100.15832805573147563No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT100.15832805573147563No Hit
GGCACTAGAATGGACCTATTCCTCATCACTAAATTATTTCAATTAAATAC100.15832805573147563No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT100.15832805573147563No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC90.14249525015832806No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAAATATTTCAATTAAATAC90.14249525015832806No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC90.14249525015832806No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA90.14249525015832806No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTAAATTATTTCAATTAAATAC90.14249525015832806No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAGTTATTTCAATTAAATAC80.1266624445851805No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAAATAAATAC80.1266624445851805No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATGC80.1266624445851805No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTCCAATTAAATAC80.1266624445851805No Hit
GGCACTAGAATGGACCTTTCCTCATCACTAAATTATTTCAATTAAATACA70.11082963901203294No Hit
GGCACTAGAATGGACCTTTTCCTCATCACTAAATTATTTCAATTAAATAC70.11082963901203294No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC70.11082963901203294No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAGATAC70.11082963901203294No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.11082963901203294No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAACTAAATAC70.11082963901203294No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAACAC70.11082963901203294No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTACTTCAATTAAATAC70.11082963901203294No Hit
GGCACTAGAATGGACCTCTCCTCATCACTAAATTATTTCAATTAAATACA70.11082963901203294No Hit
GGCACTAGAATGGACCTCTTACTCATCACTAAATTATTTCAATTAAATAC70.11082963901203294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)