FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8075
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC184622.860681114551085No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC131816.321981424148607No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC114414.1671826625387No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG103312.792569659442723No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6918.557275541795665No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2703.343653250773994No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT710.8792569659442725No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA600.7430340557275541No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG550.6811145510835914No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT300.37151702786377705No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.34674922600619196No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT210.26006191950464397No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT200.24767801857585142No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.22291021671826625No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.22291021671826625No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.21052631578947367No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.19814241486068113No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.19814241486068113No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA150.18575851393188852No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT150.18575851393188852No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA140.17337461300309598No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC140.17337461300309598No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1609907120743034No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.14860681114551083No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT120.14860681114551083No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.14860681114551083No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.13622291021671826No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC110.13622291021671826No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.13622291021671826No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.12383900928792571No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.12383900928792571No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.12383900928792571No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.12383900928792571No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.11145510835913312No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC90.11145510835913312No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.11145510835913312No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.11145510835913312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)