FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952
Total Bases283 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT68635.14344262295082No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG61631.557377049180328No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC25212.90983606557377No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC522.663934426229508No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG261.331967213114754No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.819672131147541No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC110.5635245901639344No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA110.5635245901639344No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG100.5122950819672131No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG90.4610655737704918No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTGTGTGAAGACCAAGTCTCT80.4098360655737705No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT80.4098360655737705No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.3073770491803279No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.25614754098360654No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.25614754098360654No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG50.25614754098360654No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.25614754098360654No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCGT40.20491803278688525No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT40.20491803278688525No Hit
ACTTAAAAACAAGGCAGTGCTACTGCCTAATGGTGCTACAGTTTCTGCCT40.20491803278688525No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG30.15368852459016394No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.15368852459016394No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT30.15368852459016394No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG30.15368852459016394No Hit
TGTGTGTGCGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG30.15368852459016394No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC30.15368852459016394No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT30.15368852459016394No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.15368852459016394No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.15368852459016394No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.15368852459016394No Hit
ACTTAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTCT30.15368852459016394No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.15368852459016394No Hit
ACTTAAAAACAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.10245901639344263No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTGTGTGAAGACCAAGTCTCT20.10245901639344263No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG20.10245901639344263No Hit
ACTTAAAAACAAGGCAGCGCTGAACGGTATTTGTGTGAAGACCAAGTCTC20.10245901639344263No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTATAAGGTTTGGACAGAAGGGTAAAG20.10245901639344263No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG20.10245901639344263No Hit
ACTTAAAAACAAGGCAACTCTGAACGGTATTTGTGTGAAGACCAAGTCTC20.10245901639344263No Hit
ACTTAAAAACAAGGCAGTGATAATGCCTAATGGTGCTACAGTTTCTGCCT20.10245901639344263No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTGTGTGAAGACCAAGTCTCT20.10245901639344263No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTCTGGACAGAAGGGTAAAG20.10245901639344263No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG20.10245901639344263No Hit
ACTTAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCTG20.10245901639344263No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTCTCTGCCT20.10245901639344263No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT20.10245901639344263No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCCAATGGTGCTACAGTTTCTGCCT20.10245901639344263No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGCTTGGACAGAAGGGTAAAG20.10245901639344263No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG20.10245901639344263No Hit
ACCTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC20.10245901639344263No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.10245901639344263No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGTAGGGTAAAG20.10245901639344263No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.10245901639344263No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTTTTGTGTGAAGACCAAGTCTCT20.10245901639344263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)