FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2517
Total Bases364.8 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC41816.607071911005164No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC40716.17004370282082No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG38915.454906634882798No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC31312.435439014700039No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC27711.005164878823996No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG1234.886769964243146No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT431.7083829956297178No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG271.0727056019070322No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT160.6356773937226857No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA140.5562177195073501No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.31783869686134286No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.27810885975367505No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.23837902264600713No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT60.23837902264600713No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.1986491855383393No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1986491855383393No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.1986491855383393No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1986491855383393No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1986491855383393No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1986491855383393No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.1986491855383393No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.15891934843067143No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.15891934843067143No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.15891934843067143No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.15891934843067143No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.15891934843067143No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.15891934843067143No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC40.15891934843067143No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAACTAAATAC30.11918951132300357No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCGAATTGCTGCTGGAACC30.11918951132300357No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA30.11918951132300357No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACAGAAACACACACACAACC30.11918951132300357No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.11918951132300357No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.11918951132300357No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.11918951132300357No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA30.11918951132300357No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT30.11918951132300357No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.11918951132300357No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.11918951132300357No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACAC30.11918951132300357No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.11918951132300357No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.11918951132300357No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC30.11918951132300357No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.11918951132300357No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.11918951132300357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)