FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5816
Total Bases843.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT277547.713204951856945No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC94016.162310866574966No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG77513.325309491059148No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC2644.53920220082531No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC801.3755158184319118No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG771.3239339752407153No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC651.1176066024759286No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG310.5330123796423659No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA290.498624484181568No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT260.4470426409903714No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC230.3954607977991747No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC180.30949105914718017No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.24071526822558462No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCGT130.2235213204951857No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC110.1891334250343879No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT110.1891334250343879No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC110.1891334250343879No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGCGCTACAGTTTCTGCCT100.17193947730398898No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT100.17193947730398898No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.17193947730398898No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG90.15474552957359008No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT90.15474552957359008No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.1375515818431912No Hit
ACTTAAAAACAAGGCGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.1375515818431912No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.1375515818431912No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC70.12035763411279231No Hit
ACTTAAAAACAAGGCAGTGCTAACGCCTAATGGTGCTACAGTTTCTGCCT70.12035763411279231No Hit
ACTTAAAAACAAGGCATGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.10316368638239339No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTGTGTGAAGACCAAGTCTCT60.10316368638239339No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAGTGGTGCTACAGTTTCTGCCT60.10316368638239339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)