FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457
Total Bases356.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT92037.44403744403744No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG87735.6939356939357No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC2329.442409442409442No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC562.2792022792022792No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC240.9768009768009768No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG230.9361009361009361No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC180.7326007326007326No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.3663003663003663No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.3256003256003256No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA80.3256003256003256No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC60.2442002442002442No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG50.20350020350020348No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGCGCTACAGTTTCTGCCT50.20350020350020348No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.20350020350020348No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTGCAGTTTCTGCCT50.20350020350020348No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.1628001628001628No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTATAGGGGCACTCAGA40.1628001628001628No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCGT40.1628001628001628No Hit
ACTTAAAAACAAGGCAGTACTAATGCCTAATGGTGCTACAGTTTCTGCCT40.1628001628001628No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG40.1628001628001628No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG40.1628001628001628No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.1628001628001628No Hit
TGTGTGTGTGTTTCTGTTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG40.1628001628001628No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG40.1628001628001628No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG40.1628001628001628No Hit
ACTTAAAAACAAGGCAGTGCTAACGCCTAATGGTGCTACAGTTTCTGCCT30.1221001221001221No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT30.1221001221001221No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG30.1221001221001221No Hit
TGAGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG30.1221001221001221No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTGTGTGAAGACCAAGTCTCT30.1221001221001221No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.1221001221001221No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.1221001221001221No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.1221001221001221No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGCTTGGACAGAAGGGTAAAG30.1221001221001221No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC30.1221001221001221No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCTT30.1221001221001221No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)