FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457
Total Bases356.1 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC80832.88563288563289No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC79432.31583231583232No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG2249.116809116809117No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG562.2792022792022792No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT230.9361009361009361No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG170.6919006919006919No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.6919006919006919No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC130.5291005291005291No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.4884004884004884No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.40700040700040696No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.3663003663003663No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.3663003663003663No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA80.3256003256003256No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.3256003256003256No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACC70.2849002849002849No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.2442002442002442No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.2442002442002442No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.2442002442002442No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.2442002442002442No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.2442002442002442No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.20350020350020348No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.20350020350020348No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.20350020350020348No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.20350020350020348No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.20350020350020348No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA50.20350020350020348No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAAAAC40.1628001628001628No Hit
AAATACCGTTCAGCGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC40.1628001628001628No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTAGCAAATTGCTGCTGGAACC40.1628001628001628No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT40.1628001628001628No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC40.1628001628001628No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC40.1628001628001628No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC30.1221001221001221No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC30.1221001221001221No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTCGAACC30.1221001221001221No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.1221001221001221No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC30.1221001221001221No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACT30.1221001221001221No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT30.1221001221001221No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGTTGGAACC30.1221001221001221No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.1221001221001221No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.1221001221001221No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.1221001221001221No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC30.1221001221001221No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTACTGCTGGAACC30.1221001221001221No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC30.1221001221001221No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA30.1221001221001221No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT30.1221001221001221No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)