FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885
Total Bases273.2 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC73839.15119363395225No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC47925.411140583554374No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG20911.087533156498672No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG472.493368700265252No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA201.0610079575596816No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA130.6896551724137931No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG110.583554376657825No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.5305039787798408No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.5305039787798408No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT90.47745358090185674No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.42440318302387264No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT80.42440318302387264No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.3713527851458886No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.3713527851458886No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.3183023872679045No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC60.3183023872679045No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC50.2652519893899204No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.2652519893899204No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.2652519893899204No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.2652519893899204No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.2652519893899204No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC50.2652519893899204No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.21220159151193632No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC40.21220159151193632No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.21220159151193632No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC30.15915119363395225No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAAATAAATAC30.15915119363395225No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC30.15915119363395225No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.15915119363395225No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.15915119363395225No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.15915119363395225No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.15915119363395225No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTAAATTATTTCAATTAAATAC30.15915119363395225No Hit
GGCACTAGAATGGACCTATTCCTCATCACTAAATTATTTCAATTAAATAC30.15915119363395225No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAGTTATTTCAATTAAATAC20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATCATTTCAATTAAATAC20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC20.10610079575596816No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAATC20.10610079575596816No Hit
GGCACGAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC20.10610079575596816No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT20.10610079575596816No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGTTGGAACC20.10610079575596816No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAGATTGCTGCTGGAACC20.10610079575596816No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACC20.10610079575596816No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.10610079575596816No Hit
AAATACCGTTCAGAACTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC20.10610079575596816No Hit
GGCACTGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA20.10610079575596816No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTTCAAATTGCTGCTGGAACC20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCTTCATCACTAAATTATTTCAATTAAATAC20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTAAATTATTTCAATTAAATAC20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATCTCAATTAAATAC20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTACTTCAATTAAATAC20.10610079575596816No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAGATTATTTCAATTAAATAC20.10610079575596816No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAAC20.10610079575596816No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.10610079575596816No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGTTGCTGGAACC20.10610079575596816No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA20.10610079575596816No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTAGCAAATTGCTGCTGGAACC20.10610079575596816No Hit
AAATACCGTTCAGAGCTACCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.10610079575596816No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT20.10610079575596816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)