FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496
Total Bases216.9 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC87958.7566844919786No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTC29719.852941176470587No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTGTGTGAAGACCAAGTCTC322.13903743315508No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTGTGTGAAGACCAA211.4037433155080214No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT211.4037433155080214No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCGT201.3368983957219251No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTGTGTGAAGACCAAGTCTCT140.9358288770053476No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTGTGTGAAGACCAAGTCTCT100.6684491978609626No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTGTGTGAAGACCAAGTCTCT90.6016042780748663No Hit
TGTGTGTGTGTTTCTGTGGGTCCATTCTAGTGCCGTGTGAAGACCAAGTC90.6016042780748663No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTGTGTGAAGACCAAGTCTCT70.4679144385026738No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT70.4679144385026738No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT60.4010695187165776No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTGTGTGAAGACCAAGTCTCT50.3342245989304813No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT50.3342245989304813No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTGTGTGAAGACCAAGTCTCT40.267379679144385No Hit
ACTTAAAAACAAGGCGGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC40.267379679144385No Hit
ACTTAAAAACAAGGCAGTTCTGACGGTATTTGTGTGAAGACCAAGTCTCT40.267379679144385No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGGTCCATTCTAGTGCCGT30.2005347593582888No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTGTGTGAAGACCAAGTCTCT30.2005347593582888No Hit
ACTTAAAAACAAGGCAGCTTGAACGGTATTTGTGTGAAGACCAAGTCTCT30.2005347593582888No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTGTGTGAAGACCAAGTCTCT30.2005347593582888No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCGT30.2005347593582888Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTTCTAACGGTATTTGTGTGAAGACCAAGTCTCT30.2005347593582888No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.1336898395721925No Hit
ACTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.1336898395721925No Hit
ACTTAAAAACAAGGCAGCTCTGAACTGTATTTGTGTGAAGACCAAGTCTC20.1336898395721925No Hit
ACTTAAAAACAAGGCAGTGCTACCGTATGGCAGCTCTGAACGGTATTTGT20.1336898395721925No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGATTTGTGTGAAGACCAAGTCTCT20.1336898395721925No Hit
ACTTAAAAACAAGGCAGTTTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.1336898395721925No Hit
ACTTAAAAATAAGGCAGCTCTGAACGGTATTTGTGTGAAGACCAAGTCTC20.1336898395721925No Hit
ACTTAAAAACAAGGCAGTTCTGAAGGTATTTGTGTGAAGACCAAGTCTCT20.1336898395721925No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTGTGTGAAGACCAAGTCTCT20.1336898395721925No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTGTGTGAAGCCCAAGTCTC20.1336898395721925No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTGTGTGAAGACAAAGTCTC20.1336898395721925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)