FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023
Total Bases293.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG116357.48887790410282No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG35417.498764211566982No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAACCGAATGTAGAA582.8670291646070196No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATG542.6693030153237767No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT221.0874938210578349No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT180.8897676717745922No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAC150.7414730598121602No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT150.7414730598121602No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT110.5437469105289174No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT90.4448838358872961No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAC90.4448838358872961No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTACCGAATGTAGAACCATGT80.3954522985664854No Hit
GGCACTAGAATGGACCCACAGAAACACACACACAACCGAATGTAGAACCA60.2965892239248641No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.2471576866040534No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.2471576866040534No Hit
AAAACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.2471576866040534No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.2471576866040534No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACAGAATGTAGAACCATG50.2471576866040534No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT50.2471576866040534No Hit
GGCACTAGAATGGACCTACAGAAACACACACACAACCGAATGTAGAACCA50.2471576866040534No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCCTTGTTTTTAAGTAC50.2471576866040534Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTTGTTTTTAAGTAC50.2471576866040534Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT40.1977261492832427No Hit
ACACAACCGAATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGCC40.1977261492832427Illumina Single End PCR Primer 1 (95% over 23bp)
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCCATGTACTCTG40.1977261492832427Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCCGCCTTGTTTTTAAGTACCGAATGTAGAACCATG40.1977261492832427No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT40.1977261492832427No Hit
AAATACCGTTCAAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT40.1977261492832427No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT30.14829461196243204No Hit
AAATACCGTTCAGAAACACACACACAACCGAATGTAGAACCATGTCGTCA30.14829461196243204No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTACCGAATGTAGAACATGT30.14829461196243204No Hit
AAATACCGTTCAGAGCAGCCTTGTTTTTAAGTACCGAATGTAGAACCATG30.14829461196243204No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTTGAACCATG30.14829461196243204No Hit
AAATACCGTTCAGAGCTGCCATACGGTAGCACTGCCTTGTTTTTAAGTAC30.14829461196243204No Hit
GGCACTAGAATGGACCACAGAAACACACACACAACCGAATGTAGAACCAT30.14829461196243204No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGACCCATG30.14829461196243204No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGCACCATG30.14829461196243204No Hit
AAATACCGTTCAGAGCTGCCATGTTTTTAAGTACCGAATGTAGAACCATG30.14829461196243204No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATACCGAATGTAGAACCATGT30.14829461196243204No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGACCGAATGTAGAACCATGT30.14829461196243204No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTACCGAATGTAGAACCATGT30.14829461196243204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)