FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11924
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC377331.642066420664207No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC199816.756122106675612No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC150912.655149278765515No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10598.881247903388125No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC7196.029855753102986No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGTTGCTGGAACC2712.272727272727273No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2642.214022140221402No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT950.7967125125796712No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA650.5451190875545119No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT440.36900369003690037No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.3522307950352231No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.32707145253270714No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.32707145253270714No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.31868500503186853No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG380.31868500503186853No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.2851392150285139No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.25159342502515936No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2264340825226434No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.2264340825226434No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT260.21804763502180474No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA250.20966118752096613No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.20127474002012746No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.20127474002012746No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.18450184501845018No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.18450184501845018No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.15934250251593426No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.15934250251593426No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT190.15934250251593426No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.15934250251593426No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC190.15934250251593426No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT180.15095605501509562No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA160.13418316001341832No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.13418316001341832No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.12579671251257968No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.11741026501174102No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.10902381751090237No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC120.10063737001006373No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.10063737001006373No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC120.10063737001006373No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT120.10063737001006373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)