FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11619
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC300225.83699113520957No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC238220.50090369222825No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG139111.971770376108099No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC119610.293484809363973No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC9968.572166279369997No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG4373.7610809880368365No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG900.774593338497289No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA500.4303296324984938No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.3356571133488252No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.31844392804888544No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.2581977794990963No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.2581977794990963No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT300.2581977794990963No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.24098459419915652No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.2237714088992168No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.20655822359927703No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT240.20655822359927703No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA240.20655822359927703No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.20655822359927703No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.20655822359927703No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.20655822359927703No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.19795163094930715No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.1893450382993373No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.18073844564936742No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.17213185299939754No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.17213185299939754No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.17213185299939754No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1549186676994578No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1549186676994578No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC180.1549186676994578No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.14631207504948793No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT160.13770548239951805No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA150.12909888974954814No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.12909888974954814No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT140.12049229709957826No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA130.1118857044496084No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT130.1118857044496084No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.10327911179963851No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC120.10327911179963851No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.10327911179963851No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)