FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13437
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC348225.913522363622832No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC314023.368311379028057No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG13229.838505618813723No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC12529.3175560020838No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC12239.101734018009973No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG3502.604748083649624No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1170.8707300736771602No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA610.45397038029322023No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG610.45397038029322023No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.3646647317109474No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT410.3051276326560988No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.2902433578923867No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.2753590831286745No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.26791694574681846No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.2604748083649624No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.25303267098310633No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.2455905336012503No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.23814839621939418No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.23814839621939418No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT320.23814839621939418No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.23070625883753812No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.21582198407382602No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.20837984669196996No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT280.20837984669196996No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA260.19349557192825778No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.18605343454640172No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.18605343454640172No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.17861129716454566No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.1711691597826896No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA220.16372702240083353No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.16372702240083353No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.16372702240083353No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.14884274763712138No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.14884274763712138No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.14140061025526532No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.13395847287340923No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.11907419810969709No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.11907419810969709No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA150.11163206072784104No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.10418992334598498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)