FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10476
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC226821.649484536082475No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC224521.429935089728904No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC182317.40168003054601No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG9729.278350515463918No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6616.309660175639557No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT3553.3886979763268426No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2081.9854906452844596No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG740.7063764795723558No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT550.5250095456281023No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT430.4104620084001527No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.3436426116838488No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.3150057273768614No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA320.30546009927453227No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.286368843069874No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT250.23864070255822833No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.20045819014891178No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.19091256204658266No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1718213058419244No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1718213058419244No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.15273004963726614No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.15273004963726614No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.143184421534937No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.143184421534937No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.143184421534937No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.143184421534937No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.143184421534937No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.13363879343260787No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.13363879343260787No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT140.13363879343260787No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.12409316533027873No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.12409316533027873No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.12409316533027873No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.11454753722794961No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.11454753722794961No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT110.10500190912562048No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10500190912562048No Hit
AATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG110.10500190912562048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)