FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086
Total Bases302.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC55026.36625119846596No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC47422.722914669223393No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC27613.231064237775648No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1738.293384467881113No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC1416.759348034515819No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG522.4928092042186005No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT371.7737296260786195No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG120.5752636625119847No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.4793863854266539No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.38350910834132307No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.33557046979865773No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.28763183125599234No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.28763183125599234No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.28763183125599234No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC60.28763183125599234No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.23969319271332695No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.23969319271332695No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.23969319271332695No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.23969319271332695No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC40.19175455417066153No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA40.19175455417066153No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC40.19175455417066153No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.19175455417066153No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC30.14381591562799617No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC30.14381591562799617No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.14381591562799617No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.14381591562799617No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC30.14381591562799617No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.14381591562799617No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.14381591562799617No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.14381591562799617No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTAAATTATTTCAATTAAATAC30.14381591562799617No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC30.14381591562799617No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.14381591562799617No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.14381591562799617No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC30.14381591562799617No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA30.14381591562799617No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA30.14381591562799617No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)