FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9567
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC348736.44820737953381No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC130713.661544893906136No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG118012.334065015156266No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC105110.985679941465454No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG3693.8570084666039515No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC2432.539981185324553No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG720.7525870178739417No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA640.6689662381101704No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.43900909375979935No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.3971987038779137No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.37629350893697083No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA330.3449357165255566No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT310.32403052158461376No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT310.32403052158461376No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.31357792411414237No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.31357792411414237No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.29267272917319953No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT280.29267272917319953No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.28222013170272814No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.2717675342322567No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.2404097418208425No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.22995714435037107No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.19859935193895684No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.18814675446848542No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC170.177694156998014No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT160.1672415595275426No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.1672415595275426No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT140.14633636458659977No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA140.14633636458659977No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA130.13588376711612835No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.13588376711612835No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC130.13588376711612835No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC130.13588376711612835No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC130.13588376711612835No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT130.13588376711612835No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11497857217518553No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC110.11497857217518553No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.10452597470471411No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT100.10452597470471411No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.10452597470471411No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT100.10452597470471411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)