FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6126
Total Bases888 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC183729.986940907606925No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC166527.179236043095006No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG72111.76950701926216No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC2484.048318641854391No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2303.7544890630101206No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1953.1831537708129285No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG570.930460333006856No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT450.7345739471106758No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.4897159647404506No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.3428011753183154No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA210.3428011753183154No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT210.3428011753183154No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.3101534443356187No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT180.2938295788442703No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.27750571335292196No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.2611818478615736No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.2448579823702253No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT150.2448579823702253No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.22853411687887693No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.21221025138752855No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1958863858961802No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.17956252040483187No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.17956252040483187No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.17956252040483187No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT100.1632386549134835No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC100.1632386549134835No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.14691478942213515No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT90.14691478942213515No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT80.1305909239307868No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.1305909239307868No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA80.1305909239307868No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC80.1305909239307868No Hit
AAATACCGTTCAGAGCTGCCACCAAGGGCTTGCAAATTGCTGCTGGAACC80.1305909239307868No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT80.1305909239307868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)