FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10942
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC526848.14476329738622No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10359.458965454213123No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG8247.530615975141656No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC7646.98227015170901No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5905.392067263754341No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT3573.2626576494242365No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1811.6541765673551454No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA730.6671540851763845No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA590.5392067263754341No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG490.4478157558033266No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA430.39298117346006217No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.3747029793456406No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT370.3381465911167977No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.3290074940595869No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.3015902028879547No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.3015902028879547No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT330.3015902028879547No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.2924511058307439No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.27417291171632246No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA300.27417291171632246No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2467556205446902No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC240.21933832937305794No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT210.1919210382014257No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC180.16450374702979345No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.14622555291537195No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC160.14622555291537195No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.13708645585816123No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.13708645585816123No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.12794735880095048No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTAAATTATTTCAATTAAATAC140.12794735880095048No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC130.11880826174373972No Hit
AATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG120.10966916468652897No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC110.10053006762931822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)