FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4437
Total Bases643.1 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC117226.414243858462928No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC107024.11539328375028No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC56812.801442416046878No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG3086.941627225602885No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2114.755465404552626No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2034.57516339869281No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG801.803020058598152No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGTAAATTGCTGCTGGAACC280.6310570205093531No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG280.6310570205093531No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT180.4056795131845842No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.38314176245210724No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.38314176245210724No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA120.2704530087897228No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.24791525805724587No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.225377507324769No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.2028397565922921No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.2028397565922921No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.2028397565922921No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.2028397565922921No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.1803020058598152No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.1803020058598152No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT80.1803020058598152No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.15776425512733827No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.15776425512733827No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.15776425512733827No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.15776425512733827No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC70.15776425512733827No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.15776425512733827No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC70.15776425512733827No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1352265043948614No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.1352265043948614No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC60.1352265043948614No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1352265043948614No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1352265043948614No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA60.1352265043948614No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC60.1352265043948614No Hit
AATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG60.1352265043948614No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.1126887536623845No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1126887536623845No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.1126887536623845No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA50.1126887536623845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)