FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10672
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC375435.176161919040474No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC251123.528860569715143No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC8668.114692653673163No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC7066.61544227886057No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG6506.090704647676162No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2051.9209145427286356No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1081.0119940029985006No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.4591454272863569No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.3092203898050975No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.2811094452773613No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.2717391304347826No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.26236881559220393No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.26236881559220393No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.25299850074962515No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.24362818590704646No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG260.24362818590704646No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.23425787106446777No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.21551724137931033No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.20614692653673164No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.19677661169415292No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT210.19677661169415292No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.1874062968515742No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC200.1874062968515742No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT200.1874062968515742No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1780359820089955No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.1780359820089955No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.1780359820089955No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.15929535232383807No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.15929535232383807No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT170.15929535232383807No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.14992503748125938No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC150.14055472263868066No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.13118440779610197No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC140.13118440779610197No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT130.12181409295352323No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC120.11244377811094453No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10307346326836582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)