FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5792
Total Bases839.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC161627.900552486187845No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC132522.876381215469614No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC69712.033839779005524No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT3976.8542817679558015No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG3516.060082872928177No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2884.972375690607735No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG611.0531767955801106No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.5006906077348067No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT240.4143646408839779No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG220.3798342541436464No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.2935082872928177No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.2935082872928177No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.2762430939226519No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.2762430939226519No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.25897790055248615No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.24171270718232044No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.2244475138121547No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.2244475138121547No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.2244475138121547No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.20718232044198895No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.20718232044198895No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.20718232044198895No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1899171270718232No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1899171270718232No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1899171270718232No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.17265193370165746No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.17265193370165746No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.15538674033149172No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.15538674033149172No Hit
AATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG90.15538674033149172No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.12085635359116022No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC70.12085635359116022No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA70.12085635359116022No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC60.10359116022099447No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.10359116022099447No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC60.10359116022099447No Hit
AAATACCGTTCGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG60.10359116022099447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)