FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9221
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC213723.175360589957705No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC189820.583450818783213No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG109011.820843726276976No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC106011.495499403535408No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC8469.174709901312223No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG3543.8390630083505046No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG810.878429671402234No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA400.43379243032209086No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.3904131872898818No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT350.3795683765318295No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.32534432274156816No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT280.3036547012254636No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2819650797093591No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.2819650797093591No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.26027545819325454No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT220.23858583667714997No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.2277410259190977No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.21689621516104543No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.2060514044029932No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.2060514044029932No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.2060514044029932No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.2060514044029932No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.18436178288688862No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.18436178288688862No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.18436178288688862No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.17351697212883635No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.16267216137078408No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.16267216137078408No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.1518273506127318No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14098253985467954No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT130.14098253985467954No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14098253985467954No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.14098253985467954No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA120.13013772909662727No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT120.13013772909662727No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT120.13013772909662727No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11929291833857499No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.10844810758052272No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10844810758052272No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC100.10844810758052272No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA100.10844810758052272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)