FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9222
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC314934.146605942311865No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC135914.736499674690956No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC98410.670136629798309No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG93310.117111255692908No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6707.265235306874865No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2502.710908696595099No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG530.5747126436781609No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.5638690088917806No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.3795272175233138No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.3361526783777922No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.3361526783777922No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT300.32530904359141183No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT290.3144654088050315No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.30362177401865104No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT280.30362177401865104No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.29277813923227064No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.27109086965950985No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.27109086965950985No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA240.2602472348731295No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2602472348731295No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.23855996530036866No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA220.23855996530036866No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT190.20602906094122753No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1951854261548471No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.1734981565820863No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA130.14096725222294515No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT120.13012361743656475No Hit
GGCACTAGAATGGACCTCTTCCCACCCAAACCCACAGAAACACACACACA120.13012361743656475No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11927998265018433No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11927998265018433No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11927998265018433No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10843634786380395No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10843634786380395No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC100.10843634786380395No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC100.10843634786380395No Hit
GGCACTAGAATGGACCTCTCCTCATCACTACATTATTTCAATTAAATACA100.10843634786380395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)